Hospital Information Systems represent critical infrastructure for modern healthcare delivery, yet their implementation in Ugandan hospitals faced numerous challenges. This study examined the extent of implementation and utilization of Hospital Information Systems in two major healthcare facilities in Entebbe. A cross-sectional study design was employed involving 212 healthcare workers from Entebbe Regional Referral Hospital and Dr Ronald Bata Memorial Hospital. Data was collected through structured questionnaires and analyzed using SPSS version 25.0. Chi-square tests were used to determine associations between variables at a significance level of p