The transport sector in Uganda underwent significant digital transformation with the adoption of online booking systems, revolutionizing traditional ticketing methods and passenger management. This study examined the impact of online booking systems on operational efficiency and customer satisfaction at Jaguar Bus Company in Kisoro District, Uganda. The research focused on how digital ticketing platforms influenced service delivery, revenue management, and customer experience in the competitive transport industry. The study employed a mixed-methods research design combining quantitative and qualitative approaches. A sample of 200 participants, including 150 customers, 40 company staff members, and 10 management personnel, was selected using purposive and simple random sampling techniques. Data were collected through structured questionnaires, interviews, and analysis of company operational records covering a 12-month period. Statistical analysis included descriptive statistics, correlation analysis, and paired t-tests to compare pre- and post-implementation performance metrics. The findings revealed significant improvements in operational efficiency following online booking system implementation. Average ticket processing time reduced from 8.5 minutes to 2.3 minutes (t=12.45, p